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Nucleic Acids Research, 1980, Vol. 8, No. 2 403-421
© 1980

Article

Comparative study of the interaction of polyuridylic acid with 30S subunits and 70S ribosomes of Escherichia coli

V.I. Katunin, Yu.P. Semenkov, V.I. Makhno and S.V. Kirillov

B.P.Konstantinov Nuclear Physics Institute of the Academy of Sciences of the USSR, Gatchina, Leningrad district 188350, USSR

Received October 10, 1979. Fractionated polyuridylic acid with an average chain length of 55 nucleotides forms binary complexes with 30S subunits with a stoichiometry of I:I. These complexes are heterogeneous in stability. The more stable one is characterized by an association constant K2 = 5.5xI09 M–I, and the less stable-by KI = IO6xM–I, at 20 mM Mg2+, 200 mM NH+4 and 0°C. The main reason for this heterogeneity is the presence or absence of the ribosomal protein SI in the subunits. Decrease of Mg2+ concentration down to 5 mM hardly changes the K2 values but reduction of the NH+4 concentration to 50 mM results in a 25-fold increase of K2. Association constants K2 for the stable complex, i.e. in the presence of SI protein, were measured at different temperatures ( 0 – 30°C ) and the thermodynamic parameters of binding ( {delta}H°, {delta}S°, {delta}G° ) were determined.

Analogous experiments were made with 70S ribosomes. K2 values as well {delta}H°, {delta}S°, {delta}G° appeared the same both for 30S and 70S ribosomes in all conditions examined. This is strong evidence that the 50s subunits do not contribute to the interaction of poly(U) with the complete 70S ribosomes.


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