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Nucleic Acids Research, 1980, Vol. 8, No. 20 4651-4670
© 1980

MOLECULAR BIOLOGY

Inverted repeated sequences in yeast nuclear DNA

Hannah L. Klein* and Susan K. Welch{dagger}

Department of Genetics, University of Washington Seattle, WA 98195, USA

Received August 15, 1980.

The inverted repeated sequences (foldback DNA) of yeast nuclear. DNA have been examined by electron microscopy and hydroxyapatite chromatography. Of the inverted repeat structures seen in the electron microscope, 34% were hairpins and 66% had a single stranded loop at the end of a duplex stem. The number average length of the repeat was 0.3 kb and the single stranded 1 was 1.6 kb. It is estimated that there are approximately 250 inverted repeats per haploid genome. A statistical analysis of the frequency of molecules containing multiple inverted repeats showed that these secquences are non-randomly distributed. The distribution of inverted repeats was also examined by measuring the fraction of total DNA in the foldback fraction that bound to hydroxyapatite as a function of single strand fragment size. This analysis also indicated that the inverted repeats are clustered. Renaturation kinetic analysis of isolated foldback and inverted repeat stem sequence DNA showed that these sequences are enriched for repetitive DNA.

*Current address: Department of Microbiology, The University of Chicago, Chicago, IL 60637

{dagger}Current address: Department of Zoology, The University of Washington, Seattle, WA 98195, USA


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[Abstract] [PDF]


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