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Nucleic Acids Research, 1980, Vol. 8, No. 21 4943-4954
© 1980

ENZYMOLOGY

A comparison of DNA cleavage by the restriction enzymes SalPI and PstI

Jacqueline A. Carter, Keith F. Chater, Celia J. Bruton and Nigel L. Brown

John Innes Institute Colney Lane, Norwich NR4 7UH Department of Biochemistry, University of Bristol Bristol BS8 1TD, UK

Received August 7, 1980. Methods for obtaining highly active, exonuclease-free, stable preparations of the Streptomyces albus P restriction enzyme SalPI are described. SalPI and its isoschizomer PstI (from the taxonomically distant Providencia stuartii 164) both cleave their recognition sequence (5'-CTGCAG-3') to generate fragments terminating in tetranucleotide 3' extensions whose sequence is 5'-TGCA-3'. Bacteriophage R4G2 DNA, protected against SalPI cleavage by pregrowth on S. albus P, is also protected against PstI cleavage; and total DNA of both S. albus P and P. stuartii 164 is resistant to cleavage by both enzymes.


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