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Nucleic Acids Research, 1980, Vol. 8, No. 22 5157-5168
© 1980

MOLECULAR BIOLOGY

The construction of a recombinant cDNA library representative of the po1y(A)+ mRNA population from normal human lymphocytes

Derek Woods, Julian Crampton, Berwyn Clarke and Robert Williamson

Biochemistry Department, St. Mary's Hospital Medical School, University of London London W2 1PG, UK

Received October 10, 1980. A recombinant library has been constructed using the plasmid pAT153 and double stranded cDNA prepared from normal human lymphocyte poly(A)+ RNA. Transformation conditions were optimized to yield approximately 200,000 recornbinants per µg of double stranded cDNA. Statistical analysis as well as sequence complexity analysis of the inserted sequences indicates that the cDNA library is representative of >99% of the poly(A)+ RNA present in the normal human lymphocyte.


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