Skip Navigation

This Article
Right arrow Print PDF (956K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Hamana, K.
Right arrow Articles by Zama, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hamana, K.
Right arrow Articles by Zama, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1980, Vol. 8, No. 22 5275-5288
© 1980

MOLECULAR BIOLOGY

Selective release of HMG nonhistone proteins during DNase digestion of Tetrahymena chromatin at different stages of the cell cycle

Koei Hamana* and Mistuo Zama**

*College of Medical Care and Technology, Gunma University Maebashi, Gunma 371 **Division of Chemistry, National Institute of Radiological Sciences Chiba, Chiba 260, Japan

Received August 29, 1980. The possible role of LG-l, a Tetrahymena specific HMG protein found in the macronuclear chrotnatin (Hamana, K. and Iwai, K. (1979) J. Biochem. 86, 789–794), was examined in relation to the chromatin structure. The chromatin isolated from cells synchronized at different stages of the cell cycle contained about one molecule of LG-l per nucleosome. Limited digestion of the chromatin with DNase I or micrococcal nuclease selectively released LG-l with the nucleosomal core histones and Hl remained insoluble, bound to the resistant DNA. Depending on the cell stages several types of chromatin structure were distinguished by their nuclease sensitivity. However, the chromatin at different stages exhibited the similar behavior of the LG-1 release with the nucleases as a function of the degree of chromatin solubilization. The results suggest that LG-1 proteins play a role in the chromatin organization which is rather independent of the cell stages.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.