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Nucleic Acids Research, 1980, Vol. 8, No. 22 5461-5472
© 1980

MOLECULAR BIOLOGY

Simplifications in the synthesis of short oligonucleotide blocks

Chris Broka, Toyoharu Hozumi, Rene Arentzen and Keiichi Itakura

Division of Biology, Molecular Genetics Section, City of Hope Research Institute Duarte, CA 91010, USA

Received July 25, 1980. A rapid and convenient procedure has been developed for the synthesis of fully protected mono, di and trideoxyribonucleotides utilizing an aryl phosphoroditriazolide. It affords advantages over coupling strategies employing condensing reagents, such as 2,4,6-triisopropylbenzenesulfonyl tetrazolide in preparing small oligonucleotides and is relatively free of the drawbacks inherent in other approaches using bifunctional phosphorylating reagents. In particular, the synthesis of trinucleotide blocks without purification at the dimer stage is described.


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