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Nucleic Acids Research, 1980, Vol. 8, No. 22 5473-5490
© 1980

MOLECULAR BIOLOGY

Solid-phase synthesis of polynucleotides. II. Synthesis of polythymidylic acids by the block coupling phosphotriester method

Ken-ichi Miyoshi, Tetsuo Miyake, Toyoharu Hozumi and Keiichi Itakura

Division of Biology, Molecular Genetics Section, City of Hope Research Institute Duarte, CA 91010, USA

Received July 25, 1980. Synthesis of two oligothymidylic acids, tridecamer and nonadecamer, is described by a rapid and simple solid-phase method on two kinds of polyacrylamide supports derivatized from commercially available Enzacryl Gel K-2. The syntheses were performed by the phosphotriester method using di- and trithymidylic acid blocks as the incoming 3'-phosphodiester component. High coupling yields were consistently obtained and the final product was isolated very easily by high performance liquid chromatography on Permaphase AAX.


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