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Nucleic Acids Research, 1980, Vol. 8, No. 23 5739-5751
© 1980

MOLECULAR BIOLOGY

Different base per unit length ratios exist in single-stranded RNA and single-stranded DNA

John Glass and Gail W. Wertz

University of North Carolina, Department of Bacteriology and Immunology, School of Medicine Chapel Hill, NC 27514, USA

Received May 6, 1980. A significant difference was found to exist in the number of bases per unit length of single-stranded RNA as compared to single-stranded DNA when single-stranded RNA or DNA molecules of known nucleotide sequence were measured by electron microscopy using a cytochrome spreading technique.

Using this technique, single-stranded RNA was found to have 17.5% more bases per unit of length than single-stranded DNA. These ratios were verified using four different denaturing conditions for the RNA: 80% formamide, 80% formamide plus glyoxal, 80% formamide/4M urea and 80% formamide/4M urea plus glyoxal.

Molecules ranging in size from 1541 to 5386 nucleotides were examined and the number of bases per unit length was found to vary inversely with the absolute length of the molecules. The difference in ratio of bases per micrometer was consistent when RNA and DNA molecules of the same length were compared.


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