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Nucleic Acids Research, 1980, Vol. 8, No. 23 5779-5794
© 1980


MOLECULAR BIOLOGY

The structure of the yeast ribosomal RNA genes. I. The complete nucleotide sequence of the 18S ribosomal RNA gene from Saccharomyces cerevisiae+

P.M. Rubtsov, M.M. Musakhanov, V.M. Zakharyev, A.S. Krayev, K.G. Skryabin and A.A. Bayev

Institute of Molecular Biology, Academy of Sciences of the USSR Vavilov str. 32, Moscow B-334, USSR

Received October 7, 1980.

The cloned 18 S ribosomal RNA gene from Saccharomyces cerevisiae have been sequenced, using the Maxam - Gilbert procedure. From this data the complete sequence of 1789 nucleotides of the 18 S RNA was deduced. Extensive homology with many eucaryotic as well as E.coli ribosomal small subunit rRNA (S-rRNA) has been observed in the 3'-end region of the rRNA molecule. Comparison of the yeast 18 S rRNA sequences with partial sequence data, available for rRNAs of the other eucaryotes provides strong evidence that a substantial portion of the 18 S RNA sequence has been conserved in evolution.


+For Part II of this series see Nucleic Acids Research (1980) 8, 4919–4926.


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