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Nucleic Acids Research, 1980, Vol. 8, No. 24 6189-6198
© 1980

ENZYMOLOGY

A sequence-specific endonuclease (Xmn I) from Xanthomonas manihotis

Bor-Chian Lin, Ming-Cheng Chien and Shun-Ying Lou

Institute of Botany, Academia Sinica Taipei, Taiwan 115, Taiwan

Received July 15, 1980. A type II restriction endonuclease Xmn I with a novel site specificity has been isolated from Xanthomonas manihotis. Xmn I does not cleave SV40 DNA, but cleaves {varphi}X174 DNA into three fragments, which constitute 76.61%, 18.08% and 5.31% of the total length of 5386 base pairs, and cleaves pBR322 DNA into two fragments of 55.71% and 44.29% of the entire 4362 base pairs. The nucleotide sequences around the cleavage sites made by Xmn I are not exactly homologous, but they have a common sequence of 5' GAANNNNTTC 3' according to a simple computer program analysis on nucleotide sequences of {varphi}X174 DNA, pBR322 DNA and SV40 DNA. The results suggest that the cleavage site of Xmn I is located within its recognition sequence of 5' GAANNNNTTC 3'.


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