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Nucleic Acids Research, 1980, Vol. 8, No. 3 487-506
© 1980

Articles

Sequence-specific interactions of the tight-binding I12-X86 lac repressor with non-operator DNA

Albert Schmitz and David J. Galas

Departement de Biologic Moléculaire, Université de Genève Genève 4, Switzerland

Received December 11, 1979. The tight-binding I12-X86 lac repressor binds to non-operator DNA in a sequence-specific fashion. Using the DNA of the E. coli I gene we have investigated these sequence-specific interactions and compared them to the operator binding of wild-type repressor. The specific, non-operator DNA interactions are sensitive to the inducer IPTG. One strong binding site in the I gene DNA was found to be one of two expected on the basis of their homology with the lac operator. The binding of I12-X86 repressor to this site was visualized using the footprinting technique, and found to be consistent with an operator-like binding configuration. The protection pattern extends into an adjacent sequence suggesting that two repressor tetramers are bound in tandem.


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