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Nucleic Acids Research, 1980, Vol. 8, No. 3 611-621
© 1980

Articles

Photoaddition of trimethylpsoralen as a probe for the intracellular organization of Eacherichia coli DNA

Lasley M. Hallick, Carl Veith Hanson*,+, Janice O. Cacciapuoti and John E. Hearst

Department of Microbiology and Immunology, University of Oregon Health Sciences Center Portland, OR 97201 *Department of Chemistry, University of California Berkeley, CA 94720, USA

Received November 13, 1979.

It has been previously demonstrated that photoreaction with 4,5',8-tri- methylpsoralen (trioxsalen) can be used as a probe for the in vivo structure of eucaryotic chromatin. We have used this probe to analyze the organization of intracellular Escherichia coli DNA. In contrast to eucaryotic DNA, bacterial DNA within the intact cell is not protected from saturating doses of trioxsalen photoaddition. The final level of covalently bound trioxealen upon saturation is identical to that found with purified DNA. In addition, the distribution of interstrand DNA cross-links formed by low doses of triox-salen photoadducts does not exhibit the repeating pattern that has been observed with eucaryotic nucleosomes.

+Present address: California State Department of Health Services 2151 Berkeley Way, Berkeley, CA 94704


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