Nucleic Acids Research, 1980, Vol. 8, No. 5 1043-1059
© 1980
Articles |
Molecular cloning of the actin gene from yeast Saccharomyces cerevisiae
Physiologisch-Chemisches Institut, Universität Marburg Lahnberge, 3550 Marburg/Lahn, GFR
Received November 16, 1979. Two overlapping DNA fragments from yeast Saccharomyces cerevisiae containing the actin gene have been inserted into pBR322 and cloned in E.coli. Clones were identified by hybridization to complementary RNA from a plasmid containing a copy of Dictyostelium actin mRNA. One recombinant plasmid obtained (pYA102) contains a 3.93-kb HindIII fragment, the other (pYA208) a 5.1-kb PstI fragment, both share a common 2.2-kb fragment harboring part of the actin gene. Cloned yeast actin DNA was identified by R-loop formation and translation of the hybridized actin mRNA and by DNA sequence analysis. Cytoplasmic actin mRNA has been estimated to be about 1250 nucleotides long. There is only one type of the actin gene in S.cerevisiae.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. McKane, K.-K. Wen, A. Meyer, and P. A. Rubenstein Effect of the Substitution of Muscle Actin-specific Subdomain 1 and 2 Residues in Yeast Actin on Actin Function J. Biol. Chem., October 6, 2006; 281(40): 29916 - 29928. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Tsubouchi and H. Ogawa Exo1 Roles for Repair of DNA Double-Strand Breaks and Meiotic Crossing Over in Saccharomyces cerevisiae Mol. Biol. Cell, July 1, 2000; 11(7): 2221 - 2233. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. H. Park, S. S. Koh, J. H. Chun, H. J. Hwang, and H. S. Kang Nrg1 Is a Transcriptional Repressor for Glucose Repression of STA1 Gene Expression in Saccharomyces cerevisiae Mol. Cell. Biol., March 1, 1999; 19(3): 2044 - 2050. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V Brault, M. Reedy, U Sauder, R. Kammerer, U Aebi, and C Schoenenberger Substitution of flight muscle-specific actin by human (beta)-cytoplasmic actin in the indirect flight muscle of Drosophila J. Cell Sci., January 11, 1999; 112(21): 3627 - 3639. [Abstract] [PDF]
|
|
|
|
|
|
M. Yamada, N. Hayatsu, A. Matsuura, and F. Ishikawa Y'-Help1, a DNA Helicase Encoded by the Yeast Subtelomeric Y' Element, Is Induced in Survivors Defective for Telomerase J. Biol. Chem., December 11, 1998; 273(50): 33360 - 33366. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A Bauer and R Kolling The SAC3 gene encodes a nuclear protein required for normal progression of mitosis J. Cell Sci., January 6, 1996; 109(6): 1575 - 1583. [Abstract] [PDF]
|
|
|
|
 |
|
 A. Adams, D Botstein, and D. Drubin A yeast actin-binding protein is encoded by SAC6, a gene found by suppression of an actin mutation Science, January 13, 1989; 243(4888): 231 - 233. [Abstract] [PDF]
|
|
|
|
 |
|
 Y F Tsay, J R Thompson, M O Rotenberg, J C Larkin, and J L Woolford Ribosomal protein synthesis is not regulated at the translational level in Saccharomyces cerevisiae: balanced accumulation of ribosomal proteins L16 and rp59 is mediated by turnover of excess protein. Genes & Dev., June 1, 1988; 2(6): 664 - 676. [Abstract] [PDF]
|
|
|
|
|
|
D Shortle, J. Haber, and D Botstein Lethal disruption of the yeast actin gene by integrative DNA transformation Science, July 23, 1982; 217(4557): 371 - 373. [Abstract] [PDF]
|
|