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Nucleic Acids Research, 1980, Vol. 8, No. 6 1307-1318
© 1980

Articles

Activation of an internal initiation site for protein synthesis during in vitro translation

Charles B. Lawrence*

The Salk Institute, Tumor Virology Laboratory P.O. Box 85800, San Diego, CA 92138, USA

Received January 16, 1980.

The major mRNA for adenovirus 2 polypeptide pVIII sediments at 18S as assayed by in vitro translation in the messenger-dependent rabbit reticulocyte lysate system. However, a small amount of messenger activity for pVIII sediments at about 27S, coincident with the mRNA for 100 K. Isolation and fractionation of poly(A) containing RNA following in vitro translation of 27S 100 K mRNA demonstrated the appearance of an 18S messenger activity for pVIII, which is approximately the size of the authentic mRNA for this protein. Partial degradation of 27S 100 K mRNA with alkali or ribonuclease T1 also results in activation of an 18S messenger activity for pVIII suggesting that in vitro messenger activity for pVIII associated with 27S RNA is due to degradation of 100 K mRNA during translation in the cell-free system.

*Present address C. Lawrence Department of Cell Biology Baylor College of Medicine Texas Medical Center Houston, Texas 77030


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