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Nucleic Acids Research, 1980, Vol. 8, No. 6 1383-1390
© 1980

Articles

Methylated bases in mycoplasmal DNA

Aharon Razin* and Shmuel Razin**

*Department of Cellular Biochemistry, The Hebrew University-Hadassah Medical School Jerusalem, Israel **Department of Biomembranes and Ultrastructure, The Hebrew University-Hadassah Medical School Jerusalem, Israel

Received January 14, 1980. The DNAs of four Mycoplasma and one Acholeplasma species were found to cogtain methylated bases. All of the five species contained 6-methyladenine (m6Ade), the methylated base characteristic of prokaryotic DNA. The extent of methylation of adenine residues in the mycoplasmal DNA ranged from 0.2% in Mycoplasma capricolum to about 2% in Mycoplasma arginini and Mycoplasma hyorhinis with intermediate methylation values for Mycaplasma orale and Acholeplasma laidlawii DNAs. About 5.8% of the cytosine residues in M. hyorhinis DNA were methylated also. Analysis of cell culture DNA for the presence of m6 Ade as a means for detection of contamination by mycoplasmas, and the phylogenetic implications of the finding of methylated bases in mycoplasmal DNAs are discussed.


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