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Nucleic Acids Research, 1980, Vol. 8, No. 6 1391-1404
© 1980

Articles

On the promoter complex formation rate of E.coli RNA polymerases with T7 phage DNA

B.N. Belintsev*, S.K. Zavriev** and M.F. Shemyakin***

*Institute of Molecular Biology, USSR Academy of Sciences Moscow **Institute of Physiology, Georgian SSR Academy of Sciences Tbilisi ***M.M.Shemyakin Institute of Bioorganic Chemistry. USSR Academy of Sciences Moscow, USSR

Received January 9, 1980. Influence of ionic strength on the kinetics of the promoter complex formation between E. coli RNA polymerase and T7 phage DNA was investigated using a membrane filter assay. The enzyme-promoter association rate constant was determined. It varies from 109 to 3.107 M–1 sec–1 when the ionic strength is changed from zero to 0.15 M NaCl. Basing on the theoretical analysis of experimental data obtained the model for the promoter site selection assuming the enzyme sliding along the DNA is discussed.


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