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Nucleic Acids Research, 1980, Vol. 8, No. 7 1551-1560
© 1980

Articles

DNA sequence of the E.coli trpR gene and prediction of the amino acid sequence of Trp repressor

C.K. Singleton, W.D. Roeder*, Gregg Bogosian, R.L. Somerville and H.L. Weith

Department of Biochemistry, Purdue University West Lafayette, IN 47907, USA

Received March 10, 1980.

The DNA sequence of a 1041 base pair from a BamHI fragment containing the E. coli trpR gene has been determined. With this sequence and other experimental evidence, the primary structure (88 amino acids) of the Trp repressor can be predicted. Additional features of the DNA sequence include a 22 base pair region upstream from the proposed structural gene which exhibits striking homology with trp operator, thus implying that expression of the trpR gene may be under autogenous regulation.

*Present Address Basel Institute for Immunology, Basel 5, Switzerland


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