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Nucleic Acids Research, 1981, Vol. 9, No. 10 2267-2280
© 1981

MOLECULAR BIOLOGY

Base specificity of mismatch repair in Streptococcus pneumoniae

Jean-Pierre Claverys, Vincent Méjean, Anne-Marie Gasc, Francis Galibert* and Armand-Michel Sicard

Centre de Recherche de Biochimie et de Génétique Cellulaires du C.N.R.S. 118 route de Narbonne, 31062 Toulouse Cedex *Laboratoire d'Hématologie Expérimentale Centre Hayem, Hôpital St. Louis, Paris, 75010, France

Received March 26, 1981. DNA sequence analysis was undertaken to investigate the structural basis of mutations showing different integration efficiencies in Streptococcus pneumoniae. Wild type, mutant and revertant sequences at two sites in the amiA locus were determined. It appears that markers which transform efficiently or inefficiently can result from single base pair changes. A low efficiency (LE) marker corresponds to a C:G to T:A change and a high efficiency (HE) marker to a G:C to T:A change. In the latter case, two mismatches, G/A and T/C, can exist at the heteroduplex stage in transformation; only T/C appears to be recognized by the hex system which controls transforming efficiencies in pneumococcus. Each of the recognized mismatches, T/G and C/A, which result from transitional change, and T/C appears to involve at least one pyrimidine. It is proposed that the mismatch repair system of S. pneumoniae is directed against mismatched pyrimidines. DNA sequence analysis also reveals that short deletions (33 or 34 bases long) behave as very high efficiency markers, confirming that deletions are not recognized by the hex system.


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