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Nucleic Acids Research, 1981, Vol. 9, No. 12 2753-2759
© 1981

ENZYMOLOGY

DNA methyltransferases affecting the sequence 5'LCCGG

Stefan Jentsch, Ursula Günthert and Thomas A. Trautner

Max-Planck-Institut für molekulare Genetik, Abt. Trautner Ihnestrasse 63/73, D-1000 Berlin 33, GFR

Received April 9, 1981. B. subtilis phage SPB and Moraxella sp. code for DNA methyltransferases which methylate both cytosines of the sequence 5'CCGG. Experiments using a B. subtilis strain whose DNA is sensitive to HpaII and resistant to MspI degradation, indicated that methylation of the outer C of this sequence provides protection against the restriction enzyme MspI.


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