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Nucleic Acids Research, 1981, Vol. 9, No. 12 2777-2790
© 1981

MOLECULAR BIOLOGY

Cloning of multiple copies of immunoglobulin variable kappa genes in cosmid vectors

Roberto Cattaneo*, Jack Gorski and Bernard Mach

Department of Microbiology, University of Geneva Medical School 64, Avenue de la Roseraie, 1205 Geneva, Switzerland

*Present address: Department of Microbiology, University of Heidelberg, Im Neuenheimer Feld 230, D-6900 Heidelberg, FRG

Received April 8, 1981. The possibility of cloning large segments of DNA in cosmid vectors offers distinct advantages, in particular for the study of multigene families. Large size fragments of mouse embryo DNA were successfully cloned in the cosmid pHC 79. Twelve recombinants hybridizing specifically to an immunoglobulin kappa chain variable region probe were identified. In 9 of these recombinants, the size of the insert ranges from 31 to 43 kilobases. Factors affecting the cloning efficiency of a complex mammalian genome in cosmids were studied. The stability of these recombinant cosmids and the preparation of recombinant cosmid DNA are also discussed.


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J Gorski, P Rollini, and B Mach
Somatic mutations of immunoglobulin variable genes are restricted to the rearranged V gene
Science, June 10, 1983; 220(4602): 1179 - 1181.
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