Skip Navigation

This Article
Right arrow Print PDF (1892K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Goldfarb, A.
Right arrow Articles by Burger, H.-J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Goldfarb, A.
Right arrow Articles by Burger, H.-J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1981, Vol. 9, No. 12 2791-2800
© 1981

MOLECULAR BIOLOGY

Mapping of in vitro transcription units and identification of primary transcripts of the D region of bacteriophage T4

Alexander Goldfarb and Hans-Jörg Burger

Max-Planck-Institut für Biochemie D-8033 Martinsried bei München, GFR

Received April 8, 1981. The D region of bacteriophage T4 is comprised of six closely linked genes which are situated between 161 kb and 165 kb on the T4 chromosome. We studied the transcription of these genes in vitro by using DNA templates derived from a series of deletion mutants in this region. The mixture of primary products made by Escherichia coli RNA polymerase were fractionated by gel electrophoresis into discrete RNA species. The results obtained together with the known map positions of the deletions allowed to identify four wild-type and several deletion-specific transcripts of the D region. The end points of these transcripts were approximately mapped. The results demonstrate that the D region has two promoters and two terminators, an organisation which is similar to the previously established organisation of the T4 tRNA gene cluster.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.