Nucleic Acids Research, 1981, Vol. 9, No. 14 3251-3270
© 1981
MOLECULAR BIOLOGY |
The structure of the gene for the large subunit of ribulose 1,5-bisphosphate carboxylase from spinach chloroplast DNA
Division of Plant Industry, CSIRO P.O.Box 1600, Canberra City, A.C.T. 2601, Australia
Received June 2, 1981. A cloned fragment of spinach chloroplast DNA carrying the gene for the large subunit of ribulose bisphosphate (RuBP) carboxylase has been analysed by electron microscopy of R-loops, by hybridization to Northern blots of chloroplast RNA, by SI nuclease mapping and by DNA sequencing. The transcribed region of the gene is 1690 ± 3 nucleotides long and co-linear with its mRNA. It comprises a 178–179 bp 5' untranslated sequence, a 1425 bp coding region and an 85–88 bp 3' untranslated region. The deduced sequence of the 475 amino acids of the spinach large subunit protein shows 10% divergence from that of the maize large subunit protein (1). The nucleotide sequence divergence between spinach and maize over the same coding region is 16% but in the transcribed flanking regions it is 35%. Features of the spinach chloroplast gene which resemble those of bacterial genes include a 5-base Shine-Dalgarno sequence complementary to a sequence near the 3' end of chloroplast and bacterial 16S rRNA, a promoter region partially homologous to a consensus sequence of bacterial promoters, and a transcription termination region capable of forming a typical stem and loop structure.
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