Skip Navigation

This Article
Right arrow Print PDF (2747K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Tweeten, K. A.
Right arrow Articles by Molloy, G. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tweeten, K. A.
Right arrow Articles by Molloy, G. R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1981, Vol. 9, No. 14 3307-3319
© 1981

MOLECULAR BIOLOGY

Induction of premature termination of transcription of the mouse ß-globin gene by 5,6-dichloro-1-ß-D-ribofuranosylbenzimidazole (DRB)

Kathleen A. Tweeten and George R. Molloy

School of Life and Health Sciences and Department of Chemistry, University of Delaware Newark, DE 19711, USA

Received May 20, 1981. Hybridization of pulse-labeled RNA from DRB-treated Friend cells to mouse ß-globin cDNA revealed that the appearance of ß-globin mRNA in the cytoplasm was inhibited by greater than 87%. To examine the effect of DRB (125 µM) on HnRNA synthesis, nuclear RNA was electrophoresed in methyl mercuric hydroxide gels, transferred to nitrocellulose, and hybridized with ß-globin specific probes. Full-length nuclear transcripts, while present in untreated cells, were not detected in DRB-treated cells. Using restriction enzymes, the cloned ß-globin gene was divided into fragments proceeding from the 5' gene region to the 3' gene region. RNA labeled in vitro by transcription in nuclei isolated from DRB-treated cells hybridized only to the promoter proximal DNA fragment. Transcripts hybridizing to fragments from both the 5' and 3' regions of the gene were produced in nuclei from untreated cells. Together these results indicate that DRB causes premature termination of transcription within the ß-globin gene.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Genes Dev.Home page
T. Trimborn, J. Gribnau, F. Grosveld, and P. Fraser
Mechanisms of developmental control of transcription in the murine alpha - and beta -globin loci
Genes & Dev., January 1, 1999; 13(1): 112 - 124.
[Abstract] [Full Text]

Home page
 Genes Dev.Home page
H. S.Y. Mancebo, G. Lee, J. Flygare, J. Tomassini, P. Luu, Y. Zhu, J. Peng, C. Blau, D. Hazuda, D. Price, et al.
P-TEFb kinase is required for HIV Tat transcriptional activation in vivo and in vitro
Genes & Dev., October 15, 1997; 11(20): 2633 - 2644.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.