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Nucleic Acids Research, 1981, Vol. 9, No. 14 3321-3334
© 1981

MOLECULAR BIOLOGY

Nucleotide sequences of Acanthamoeba castellanii 5S and 5.8S ribosomal ribonucleic acids: phylogenetic and comparative structural analyses

Ron M. MacKay and W. Ford Doolittle

Department of Biochemistry, Faculty of Medicine, Dalhousie University Halifax, Nova Scotia B3H 4H7, Canada

Received May 20, 1981. Sequences of 5S and 5.8S rRNAs of the amoeboid protist Acanthamoeba castellanii have been determined by gel sequencing of terminally-labelled RNAs which were partially degraded with chemical reagents or ribonucleases. The sequence of the 5S rRNA is GGAUACGGCFormulaAUACUGCFormulaGAAAGCACCFormulaCUUCCCAUCFormulaGAACAGCG AFormulaGUUAAGCUGFormulaGCCAGGCGGFormulaGUUAGUACUFormulaGGGUGGGCGFormulaCCACCCGGGFormulaAUCCACCGUFormulaCCGUAUCCU0H. This sequence is compared to eukaryotic 5S rRNA sequences previously published and fitted to a secondary structure model which incorporates features of several previously proposed models. All reported eukaryotic 5S rRNAs fit this model. The sequence of the 5.8S rRNA is AAC{varphi}CCUAAFormulaAACmGGA{varphi}AUFormulaUUGGUUCUCFormulaCGAG GAUGAFormulaGAmACGCAGCFormulaAAAUGCGAUFormulaCGUAGUGUGFormulaAUCGCAGGGFormulaUCAGUGAAmUFormulaA{varphi}CGAAUCUSFormulaUGAACGCA AFormulaUUGCGCUCUFormulaGUGGUUUAAFormulaCCCCCGGGAFormulaCACGUUCGCFormulaUGAGUGCCGFormulaU(U)0H. This sequence does not fit parts of existing secondary structure models for 5.8S rRNA, and we question the significance of such models.


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C. E. BULLERWELL, M. N. SCHNARE, and M. W. GRAY
Discovery and characterization of Acanthamoeba castellanii mitochondrial 5S rRNA
RNA, March 1, 2003; 9(3): 287 - 292.
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