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Nucleic Acids Research, 1981, Vol. 9, No. 14 3365-3377
© 1981

MOLECULAR BIOLOGY

A promoter of pBR322 activated by cAMP receptor protein

Cary Queen and Martin Rosenberg

Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health Bethesda, MD 20205, USA

Received May 5, 1981. We have demonstrated in vitro the existence on the plasmid pBR322 of a promoter signal that is strictly dependent on cAMP and its receptor protein CRP. Transcription initiates with pppG at nucleotide 2270 and proceeds counterclockwise on the standard pBR322 map. DNase protection studies show that CRP selectively binds to the –35 region of the promoter. This region exhibits strong structural homologies to the binding sites of other CRP-dependent promoters.


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