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Nucleic Acids Research, 1981, Vol. 9, No. 15 3647-3656
© 1981

MOLECULAR BIOLOGY

Oligonucleotide directed mutagenesis of the human ß-globin gene: a general method for producing specific point mutations in cloned DNA

R. Bruce Wallace, Monica Schold, Merrie Jo Johnson, Piotr Dembek* and Keiichi Itakura

City of Hope Research Institute, Division of Biology, Molecular Genetics Section Duarte, CA 91010, USA *UAM Instytut Chemii Poznan, Poland

Received May 15, 1981. A nonadecanucleotide has been used both as a site specific mutagen to introduce an T->-A transversion mutation in the human ß-globin gene cloned in pBR322 as well as a probe to screen transformed colonies for the desired mutant. The specificity of the oligonucleotide as a mutagen and as a hybridization probe provide a general method for producing site specific mutations in DNA cloned in plasmid vectors such as pBR322.


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