Skip Navigation

This Article
Right arrow Print PDF (2667K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (48)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Lie-Injo, L. E.
Right arrow Articles by Kan, Y. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lie-Injo, L. E.
Right arrow Articles by Kan, Y. W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1981, Vol. 9, No. 15 3707-3717
© 1981

MOLECULAR BIOLOGY

Two types of triplicated a-globin loci in humans

Luan Eng Lie-Injo*, Alejandro R. Herrera* and Yuet Wai Kan**

*Department of Epidemiology and International Health, University of California, San Francisco, CA 94143, USA **Howard Hughes Medical Institute and Department of Medicine, University of California San Francisco, CA 94143, USA

Received April 28, 1981. DNA from healthy Malaysian newborns was studied on gene maps after digestion with different restriction endonucleases. Of 65 newborns, two were found to be carriers of two different variants of triplicated {alpha}-globin loci. In variant no. 1, found in a Malay, the three {alpha}-globin genes are in an elongated DNA fragment on digestion with Eco RI and BamHI. The third {alpha}-globin gene was found in an additional 3.7-kb fragment on digestion with Hpa I, Bgl II and Hind III. In variant no. 2, a newtype of triplicated {alpha}-globin loci, found in a Chinese, the three {alpha}-globin genes reside in an elongated DNA fragment longer than that of variant no. 1on digestion with Eco RI and Bam HI. The third {alpha}-globin gene was found inan additional 4.2-kb fragment on digestion with Hpa I and Hind III. Digestion of this variant DNA with Bgl II produced an abnormal 16.7-kb fragment in addition to the normal 7.0-kb Bgl-II fragment. The locations of the restriction sites in the two types of triplicated {alpha}-globin lociare compatible with a mechanism of unequal crossing over following two different modes of misalignment.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.