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Nucleic Acids Research, 1981, Vol. 9, No. 15 3747-3764
© 1981

MOLECULAR BIOLOGY

Two distinct intervening sequences in different ribosomal DNA repeat units of Sciara coprophila

Renate Renkawitz-Pohl*, Lloyd Matsumoto** and Susan A. Gerbi**,{dagger}

*Institut für Molekulare Genetik, Im Neuenheimer Feld 230, Universität Heidelberg 6900 Heidelberg, GFR **Division of Biology and Medicine, Brown University Box G, Providence, RI 02912, USA

{dagger}To whom all correspondence and reprint requests should be sent

Received March 31, 1981. We have prepared a partial gene library of sheared DNA from the fungus fly, Sciara coprophila, by dA-T tailing and insertion into pBR322. Two ribosomal DNA clones which differ from the usual ribosomal DNA organization in this organism were studied in detail. Clone pBc 1L–1 has an intervening sequence of 1.4 kb, and clone pBc 6D–6 has an intervening sequence of 0.9 kb. These intervening sequences occur in about the same position in 28S rDNA, but do not appear to share sequence homology with one another. Previously we found that 90% of Sciara ribosomal DNA is homogenous and lacks an intervening sequence, and our present data explains the size heterogeneity found in most of the remaining 10%. We have found no evidence of size heterogeneity in the nontranscribed spacer.


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