Skip Navigation

This Article
Right arrow Print PDF (1839K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Bienz, M.
Right arrow Articles by Grosjean, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bienz, M.
Right arrow Articles by Grosjean, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1981, Vol. 9, No. 15 3835-3850
© 1981

MOLECULAR BIOLOGY

Usage of the three tennination codons in a single eukaryotic cell, the Xenopus laevis oocyte

M. Bienz1, E. Kubli1, J. Kohli2, S. deHenau3, G. Huez3, G. Marbaix3 and H. Grosjean3

1Zoological Institute, University of Zürich-Irchel Winterthurerstrasse 190, CH-8057 Zürich, Switzerland 2Institute of General Nicrobiology, University of Berne Altenbergrain 21, CH-3013 Bern, Switzerland 3Laboratoires de Chimie Biologique, Université de Bruxelles 67, Rue des Chevaux, B-1640 Bruxelles, Belgium

Received May 5, 1981. Oocytes from Xenopus laevis were injected with purified amber (UAG), ochre (UAA), and opal (UGA) suppressor tRNAs from yeasts. The radioactively labeled proteins translated from the endogeneous mRNAs were then separated on two–dimensional gels. All three termination codons are used in a single cell, the Xenopus laevis oocyte. But a surprisingly low number of readthrough polypeptides were observed from the 500 mRNAs studied in comparison to uninjected oocytes. The experimental data are compared with the conclusions obtained from the compilation of all available termination sequences on eukaryotic and prokaryotic mRNAs. This comparison indicates that the apparent resistance of natural termination codons against readthrough, as observed by the microinjection experiments, cannot be explained by tandem or very close second stop codons. Instead it suggests that specific context sequences around the termination codons may play a role in the efficiency of translation termination.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
M. Buvoli, A. Buvoli, and L. A. Leinwand
Suppression of Nonsense Mutations in Cell Culture and Mice by Multimerized Suppressor tRNA Genes
Mol. Cell. Biol., May 1, 2000; 20(9): 3116 - 3124.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.