Skip Navigation

This Article
Right arrow Print PDF (425K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Van Charldorp, R.
Right arrow Articles by Hilbers, C.W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Van Charldorp, R.
Right arrow Articles by Hilbers, C.W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1981, Vol. 9, No. 17 4413-4422
© 1981

MOLECULAR BIOLOGY

Destabilization of secondary structure in 16S ribosomal RNA by dimethylation of two adjacent adenosines

R. Van Charldorp, H.A. Heus, P.H.Van Knippenberg, J. Joordens, S.H. De Bruin and C.W. Hilbers

Department of Biochemistry, State University of Leiden P.O. Box 9505, 2300 RA Leiden, The Netherlands Department of Biophysical Chemistry, Catholic University Toerooiveld, 6525 ED Nijmegen, The Netherlands

Received June 24, 1981. Fragments comprising the 49 nucleotides from the 3'-end have been purified from 16s ribosomal RNA of wild-type Esoheriohia coli and from a kasugamycin-resistant mutant that specifically lacks dimethylation of two adjacent adenines near the 3'-terminus. These fragments, obtained after treatment of ribosomes in vitro with the bacteriocin cloacin DF13, were used to study the effect of the methylgroups on the temperature dependent unfolding of double-stranded regions. Both fragments contain at least 3 independent melting transitions, of which the one with the highest Tm corresponds with the unfolding of a nine-basepair long central hairpin. Dimethylation of the adenines in the loop of this hairpin lowers the melting temperature (Tm) by approximately 2°C at 0.2 M NaCl and by about 5°C at 0.015 M NaCl. It is suggested that m26Am26A is more antagonistic to loop formation than ApA and that the function of the methylgroups is to help to destabilize the 3'-terminal hairpin in 16S rRNA in order to facilitate intermolecular interactions.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.