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Nucleic Acids Research, 1981, Vol. 9, No. 19 4801-4812
© 1981

MOLECULAR BIOLOGY

Transcription of T-DNA in octopine and nopaline crown gall tumours is inhibited by low concentrations of {alpha}-amanitin

L. Willmitzer, W. Schmalenbach and J. Schell

Max-Planck-Institut für Züchtungsforschung, Abteilung Schell D-5000 Köln 30, GFR

Received August 10, 1981. Highly purified and physiologically active nuclei were isolated from four different octopine and nopaline crown gall lines. These nuclei exhibited a high endogenous RNA synthesizing activity involving all three RNA-polymerases I, II and III. Isolated nuclei were shown by Southern blotting to synthesize T-DNA specific RNA. This synthesis was shown to be sensitive to actinomycin D and therefore to be DNA-dependent. The transcription of the T-DNA was also inhibited for more than 90% by low concentrations of {alpha}-amanitin (0.7 µg/ml) indicating that the T-DNA, although from bacterial origin, is transcribed by the host RNA polymerase II.


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