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Nucleic Acids Research, 1981, Vol. 9, No. 19 4833-4845
© 1981

ENZYMOLOGY

Clal, a new restriction endonuclease from Caryophanon latum L

H. Mayer*, R. Grosschedl+, H. Schütte* and G. Hobom+

*Gesellschaft für Biotechnologische Forschung mbH Mascheroder Weg 1, D-3300 Braunschweig +Institut für Biologie III der Universität Freiburg Schänzlestrasse 1, D-7800 Freiburg, GFR

Received July 27, 1981. From Caryophanon latum L a site specific restriction endonuclease (ClaI) has been purified, which recognises the DNA hexanucleotide palindrome Formula. Staggered cleavage generates DNA restriction fragments with 5'-terminal pCG extensions. A ClaI map of bacteriophage {lambda} has been determined, which indicates cleavage inhibition due to adenine methylation at overlapping ClaI-GATC recognition sequences. Plasmid pBR322 is cut only once, in the tetracycline promoter region, and can, therefore, be used as a vector system for cloning fragments derived from ClaI digestions, and in addition for fragments generated by TaqI, HpaII, and several other enzymes.


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