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Nucleic Acids Research, 1981, Vol. 9, No. 19 4895-4908
© 1981

MOLECULAR BIOLOGY

There are approximately 20 actin genes in the human genome

S. E. Humphries1, R. Whittall1, A. Minty2, M. Buckingham2 and R. Williamson1

1Department of Biochemistry, St. Mary's Hospital Medical School, University of London London W2 1PG, UK 2Department of Molecular Biology, Institut Pasteur 25 rue du Docteur Roux, 75015 Paris, France

Received July 23, 1981. By three different lines of evidence there are approximately 20 copies of actin genes in the human genome. Firstly, the rate of hybridisation of a mouse actin probe to human DNA indicates that there are a minimum of 20 complementary copies of the actin sequence per genome. Secondly, this probe hybridises to 17–20 bands in Southern blots of restriction enzyme digests of total human DNA. Most of these bands hybridise with both 3' and 5' fragments of the cDNA and are therefore likely to contain the entire gene sequence. Thirdly, we have picked 12 actin recombinants from a genomic library, and at the level of restriction enzyme mapping these represent nine different genes. Probability calculations indicate that these recombinants were picked from a pool of at least 20 different genes.


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