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Nucleic Acids Research, 1981, Vol. 9, No. 19 5175-5184
© 1981

MOLECULAR BIOLOGY

Lac repressor - lac operator interaction. Circular dichroism study

F. Culard and J.C. Maurizot

Centre de Biophysique Moléculaire C.N.R.S., 1A, avenue de la Recherche Scientifique, 45045 Orléans Cedex, France

Received May 27, 1981. The interaction between lac repressor and a small operator DNA fragment have been examined by circular dichroism spectroscopy. The binding of lac represser on the operator induces a conformational change of the DNA which is different from that observed upon non specific binding on non operator DNA. The CD titration curve indicates that the stoechiometry of interaction is complex. A two operators-one repressor complex was found. This result was confirmed by a gel filtration experiment.


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