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Nucleic Acids Research, 1981, Vol. 9, No. 20 5297-5310
© 1981

MOLECULAR BIOLOGY

Genomic organization and transcription of the {alpha}ß heat shock DNA in Drosophila melanogaster

John T. Lis*, David Ish-Horowicz{dagger} and Sheena M. Pinchin{dagger}

*Section of Biochemistry, Molecular and Cell Biology, Wing Hall, Cornell University Ithaca, NY 14853, USA {dagger}Imperial Cancer Research Fund, Mill Hill Laboratories Burtonhole Lane, London NW7 1AD, UK

Received July 13, 1981. Previous studies have shown that (i) several RNAs induced by heat shock of Drosophila melanogaster cells are homologous to tandemly repeated {alpha}ß units found in cloned segments of D. melanogaster DNA, and (ii) the {alpha}ß sequences are present both at a major heat shock locus, 87C1, and the chromocenter of polytene chromosomes (Lis, J.T., Prestidge, L. and Hogness, D.S. [1978] Cell 14, 901–919). We have used deficiencies that delete DNA from the 87C region to examine the arrangement of {alpha}ß sequences at this locus and in the centromeric heterochromatin that comprises the chromocenter, and also to determine the chromosomal location of the induced transcription. The tandemly repeated {alpha}ß units are restricted to the 87C locus. In contrast, the chromocentral {alpha}ß sequences do not form intact {alpha}ß units, and are dispersed at heterochromatic sites in some other form. Although only half of the {alpha}ß DNA is at the 87c locus, essentially all {alpha}ß transcripts (>99.5%) are derived from this locus.


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