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Nucleic Acids Research, 1981, Vol. 9, No. 22 5867-5882
© 1981

MOLECULAR BIOLOGY

The initiator tRNA genes of Drosophila melanogaster: evidence for a tRNA pseudogene

S. Sharp, D. DeFranco, M. Silberklang*, H.A. Hosbach*, T. Schmidt**, E. Kubli**, J.P. Gergen***, P.C. Wensink*** and D. Söll

Department of Molecular Biophysics and Biochemistry, Yale University New Haven, CT 06511, USA *Department of Biochemistry and Biophysics, University of California San Franscisco, CA 94143, USA **Zoologisches Institut der Universität, Zürich Winterthurerstrasase 190, 8057 Zürich, Switzerland ***Department of Biochemistry and Rosenstiel Basic Medical Sciences Research Center, Brandeis University Waltham, MA 02254, USA

Received October 6, 1981. We have isolated four segments of Drosophila melanogaster DNA that hybridize to homologous initiator tRNAMet. Three of the cloned fragments contain initiator tRNA genes, each of which can be transcribed in vitro. The fourth clone, pPW568, contains an initiator tRNA pseudogene which is not transcribed in vitro by RNA polymerase III. The pseudogene is contained in a 1.15 kb DNA fragment. This fragment has the characteristics of dispersed repetitive DNA and hybridizes in situ to at least 30 sites in the Drosophila genome. The arrangement or the initiator tRNA genes we have isolated, is different to that of other Drosophila tRNA gene families. The initiator tRNA genes are not clustered nor intermingled with other tRNA genes. They occur as single copies within an approximately 415-bp repeat segment which is separated from other initiator tRNA genes by a mean distance of 17 kb. In situ hybridization to polytene chromosomes localizes these genes to the 61D region of the Drosophila genome. Hybridization analysis of genomic DNA indicates the presence of 8-9 non-allelic initiator tRNA genes in Drosophila melanogaster.


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