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Nucleic Acids Research, 1981, Vol. 9, No. 22 6031-6046
© 1981

MOLECULAR BIOLOGY

The interaction of RNA polymerase II with non-promoter DNA sites

D.Walt Chandler and Jay Gralla

Biochemistry Division of the Chemistry Department, and Molecular Biology Institute, University of California 405 Hilgard Avenue, Los Angeles, CA 90024, USA

Received October 20, 1981. Various complexes formed between purified RNA polymerase II and simian virus 40 DNA have been characterized with respect to rates of formation, rates of dissociation, and initial velocity of RNA synthesis. Two different types of complexes can form on intact DNA templates. One of these is formed rapidly, but is quite labile; the other forms more slowly, but is moderately stable once formed. The introduction of a single strand break into DNA leads to rapid and stable complex formation, and thus is expected to create the favored binding site. The observed properties of these complexes provide a general framework for describing the interactions of RNA polymerase II at non-promoter DNA sites. This framework appears to be similar to that established for Escherichia coli RNA polymerase interactions, suggesting that the fundamental mode of non-promoter DNA binding is similar for the bacterial, plant, and mammalian enzymes.


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