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Nucleic Acids Research, 1981, Vol. 9, No. 23 6305-6322
© 1981

MOLECULAR BIOLOGY

Sequences at the capped 5'-ends of polyoma virus late region mRNAs: an example of extreme terminal heterogeneity

Alison Cowie, Chiara Tyndall and Robert Kamen

Transcription Laboratory, Imperial Cancer Research Fund Laboratories Lincoln's Inn Fields, London WC2A 3PX, UK

Received October 12, 1981. We have localized with respect to the genomic DNA sequence the capped 5'-termini of polyoma virus late region mRNAs. A minimum of fifteen different purine termini were found within a 94 base pair region (66.36 to 68.12 map units, nt 5075-5168) immediately preceding the sequence determining the late region mRNA leader repeat (1-3). The most common termini occur at nearly every possible purine within a 25 bp sequence proximal to the leader repeat unit. These do not bear the usual positional relationship to a sequence resembling the ‘TATA’ box consensus. Deletion mutants lacking minor cap sites and sequences upstream from the principal cap sites were viable. A deletion mutant lacking one of the principal cap sites formed small plaques, while a slightly larger deletion further impinging on the principal cap site region was non-viable. The principal cap sites, which we assume to be transcriptional initiation points, are included in a DNaseI hypersensitive region of polyoma virus chromatin (4).


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