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Nucleic Acids Research, 1981, Vol. 9, No. 23 6457-6469
© 1981

MOLECULAR BIOLOGY

5S Ribosomal RNA genes of the newt Notophthalmus viridescens

Brian K. Kay* and Joseph G. Gail

Department of Biology, Yale University New Haven, CT 06511, USA

Received September 1, 1981.

The genes which code for the 5S ribosomal RNA in the newt, Notophthalmns viridescens have been cloned and analyzed. Two types of repeating unit were detected: a major type consisting of a 120 bp coding region with a 111 bp spacer, and a minor type composed of a coding region, a pseudogene, and a 113 bp spacer. The pseudogene is a 36 bp segment which corresponds to the 3' terminal third of the 5S RNA gene, and is situated immediately 3' to the gene, being separated from it by 2 bp. Two recombinant plasmids were obtained in which the major and minor units were arranged in an interspersed pattern.

*Current address: Labortory of Biochemistry, National Cancer Institute, Bethesda, MD 20205, USA


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