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Nucleic Acids Research, 1981, Vol. 9, No. 24 6953-6958
© 1981

MOLECULAR BIOLOGY

The structure of the yeast ribosomal RNA genes. 4. Complete sequence of the 25 S rRNA gene from Saccharomyces cerevisiae

O.I. Georgiev, N. Nikolaev, A.A. Hadjiolov, K.G. Skryabin, V.M. Zakharyev and A.A. Bayev

Institute of Molecular Biology, Bulgarian Academy of Sciences 1113 Sofia, Bulgaria Institute of Molecular Biology, Academy of Sciences of the USSR Vavilov str. 32, Moscow B-312, USSR

Received October 19, 1981. The complete nucleotide sequence of the 25 5 rRNA gene from one rDNA repeating unit of Saccharomyces cerevisiae has been determined. The corresponding 25 S rRNA molecule contains 3392 nucleotides,and has an estimated relative molecular mass ( Mr, Na-salt) of 1.17 x 106. Striking sequence homology is observed with known 5'- and 3'-end terminal segments of L-rRNA from other eukaryotes. Possible models of interaction with 5.8 S rRNA are discussed.


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