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Nucleic Acids Research, 1981, Vol. 9, No. 3 623-631
© 1981

MOLECULAR BIOLOGY

Identification of a protein coded by pR plasmid and involved in SOS repair in E. coli

Franca Gigliani+, Raffaella Eili+, Liana Marcucci+ and Piero A. Battaglia{dagger}

+Istituto di Biologia generale Il, Università di Roma Roma, Italy {dagger}Istituto Superiore di Sanità Roma Italy

Received October 28, 1980. The TP120 plasmid is known to determine enhanced UV sur-vival in E. coli wild type and uvrB and PolA mutants but notin RecA mutant.

In order to analyze the function involved in the SOS re-pair, we have constructed a new plasmid named pR derived bycleavage of TP120 with Hind III endonuclease. This new plasmidmaintains the Ap and UV resistance. The insertion of Tn5 trans-poson in the plasmid allows to select several pR::Tn5 plasmidswhose UV resistance was inactivated by the transposition. Thecomparison of the protein synthesis in the minicells of the pRand pR::Tn5 shows that the pR codes for a 22.000 M.W. daltonprotein which is absent in protein pattern of pR::Tn5.


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