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Nucleic Acids Research, 1981, Vol. 9, No. 6 1519-1530
© 1981

CHEMISTRY

A chemical method to enrich RNA by molecules having 5'-terminal triphosphate groups

P.M. Chumakov*, M.A. Grachev**, S.V. Netesov+,+ and I.N. Shatsky{dagger}

* Institute of Molecular Biology, USSR Academy of Sciences Moscow ** Novosibirsk Institute of Organic Chemistry, Siberian Branch of the Academy of Sciences of the USSR Novosibirsk +All-Union Scientific-Research Institute of Molecular Biology Novosibirsk, 90, USSR {dagger} Belozersky Interfaculty Laboratory of Molecular Biology and Bioorganic Chemistry of the Lomonosov Moscow State University Moscow

+ To whom requests for reprints should be sent

Received November 20, 1980. A method is proposed to enrich RNA molecules having a 5'-terminal triphosphate group. The method is based upon selective chemical modification of 5'-triphosphate groups by an antigen-containing amine followed by affinity chromatography on an absorbent loaded with antibodies specific to this antigen. A purification factor up to 37 may be achieved.


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