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Nucleic Acids Research Advance Access published online on March 16, 2007
Nucleic Acids Research, doi:10.1093/nar/gkl1137
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© 2007 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Molecular Biology

The L1Tc non-LTR retrotransposon of Trypanosoma cruzi contains an internal RNA-pol II-dependent promoter that strongly activates gene transcription and generates unspliced transcripts

Sara R. Heras, Manuel C. López*, Mónica Olivares1 and M. Carmen Thomas*

Departamento de Biología Molecular, Instituto de Parasitología y Biomedicina ‘López Neyra’, CSIC, 18001 Granada, Spain

*To whom correspondence should be addressed. Tel: +34 958 181 662; Fax: +34 958 181 632; Email: mclopez{at}ipb.csic.es Correspondence may also be addressed to M. Carmen Thomas. Tel: +34 958 181 662; Fax: +34 958 181; Email: mcthomas{at}ipb.csic.es

Received August 30, 2006. Revised December 7, 2006. Accepted December 14, 2006.

L1Tc is the best represented autonomous LINE of the Trypanosoma cruzi genome, throughout which several functional copies may exist. In this study, we show that the first 77 bp of L1Tc (Pr77) (also present in the T. cruzi non-autonomous retrotransposon NARTc, in the Trypanosoma brucei RIME/ingi elements, and in the T. cruzi, T. brucei and Leishmania major degenerate L1Tc/ingi-related elements [DIREs]) behave as a promoter element that activates gene transcription. The transcription rate promoted by Pr77 is 10–14-fold higher than that mediated by sequences located upstream from the T. cruzi tandemly repeated genes KMP11 and the GAPDH. The Pr77 promoter-derived mRNAs initiate at nucleotide +1 of L1Tc, are unspliced and translated. L1Tc transcripts show a moderate half life and are RNA pol II dependent. The presence of an internal promoter at the 5' end of L1Tc favors the production of full-length L1Tc RNAs and reinforces the hypothesis that this mobile element may be naturally autonomous in its transposition.

1Present address: PulevaBiotech, Camino Purchil 66, 18004 Granada, Spain


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