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Nucleic Acids Research Advance Access published online on August 25, 2006
Nucleic Acids Research, doi:10.1093/nar/gkl452
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© 2006 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Unraveling helicase mechanisms one molecule at a time

Ivan Rasnik1, Sua Myong2 and Taekjip Ha2,3,*

1 Department of Physics, Emory University Atlanta, GA 30322,USA 2 Department of Physics, University of Illinois Urbana, IL 61801, USA 3 Howard Hughes Medical Institute Urbana, IL 61801, USA

*To whom correspondence should be addressed. Tel: +1 271 265 0717; Email: tjha{at}uiuc.edu

Received April 1, 2006. Revised May 25, 2006. Accepted June 12, 2006.

Recent years have seen an increasing number of biological applications of single molecule techniques, evolving from a proof of principle type to the more sophisticated studies. Here we compare the capabilities and limitations of different single molecule techniques in studying the activities of helicases. Helicases share a common catalytic activity but present a high variability in kinetic and phenomenological behavior, making their studies ideal in exemplifying the use of the new single molecule techniques to answer biological questions. Unexpected phenomena have also been observed from individual molecules suggesting extended or alternative functionality of helicases in vivo.


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