Skip Navigation



Nucleic Acids Research Advance Access published online on February 1, 2007
Nucleic Acids Research, doi:10.1093/nar/gkl831
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (838K) Freely available
Right arrow Screen PDF (840K) Freely available
Right arrowOA All Versions of this Article:
35/4/1367    most recent
gkl831v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Weinert, B. T.
Right arrow Articles by Rio, D. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Weinert, B. T.
Right arrow Articles by Rio, D. C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Structural Biology

DNA strand displacement, strand annealing and strand swapping by the Drosophila Bloom's syndrome helicase

Brian T. Weinert and Donald C. Rio*

Department of Molecular and Cell Biology, 16 Barker Hall #3204, University of California Berkeley, CA 94720-3204, USA

*To whom correspondence should be addressed. Tel: +1 510 642 1071; Fax: +1 510 642 6062; Email: don_rio{at}berkeley.edu

Received July 19, 2006. Revised October 3, 2006. Accepted October 8, 2006.

Genetic analysis of the Drosophila Bloom's syndrome helicase homolog (mus309/DmBLM) indicates that DmBLM is required for the synthesis-dependent strand annealing (SDSA) pathway of homologous recombination. Here we report the first biochemical study of DmBLM. Recombinant, epitope-tagged DmBLM was expressed in Drosophila cell culture and highly purified protein was prepared from nuclear extracts. Purified DmBLM exists exclusively as a high molecular weight (~1.17 MDa) species, is a DNA-dependent ATPase, has 3'->5' DNA helicase activity, prefers forked substrate DNAs and anneals complementary DNAs. High-affinity DNA binding is ATP-dependent and low-affinity ATP-independent interactions contribute to forked substrate DNA binding and drive strand annealing. DmBLM combines DNA strand displacement with DNA strand annealing to catalyze the displacement of one DNA strand while annealing a second complementary DNA strand.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
K. Kikuchi, H. I. Abdel-Aziz, Y. Taniguchi, M. Yamazoe, S. Takeda, and K. Hirota
Bloom DNA Helicase Facilitates Homologous Recombination between Diverged Homologous Sequences
J. Biol. Chem., September 25, 2009; 284(39): 26360 - 26367.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
F. Hartung, S. Suer, and H. Puchta
Two closely related RecQ helicases have antagonistic roles in homologous recombination and DNA repair in Arabidopsis thaliana
PNAS, November 20, 2007; 104(47): 18836 - 18841.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. McVey, S. L. Andersen, Y. Broze, and J. Sekelsky
Multiple Functions of Drosophila BLM Helicase in Maintenance of Genome Stability
Genetics, August 1, 2007; 176(4): 1979 - 1992.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.