Skip Navigation



Nucleic Acids Research Advance Access published online on November 16, 2006
Nucleic Acids Research, doi:10.1093/nar/gkl903
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (3775K) Freely available
Right arrow Screen PDF (871K) Freely available
Right arrowOA All Versions of this Article:
34/21/6362    most recent
gkl903v3
gkl903v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Choudhury, N. R.
Right arrow Articles by Mukherjee, S. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Choudhury, N. R.
Right arrow Articles by Mukherjee, S. K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2006 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Molecular Biology

The oligomeric Rep protein of Mungbean yellow mosaic India virus (MYMIV) is a likely replicative helicase

Nirupam Roy Choudhury, Punjab Singh Malik, Dharmendra Kumar Singh, Mohammad Nurul Islam, Kosalai Kaliappan and Sunil Kumar Mukherjee*

Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology Aruna Asaf Ali Marg, New Delhi-110 067, India

*To whom correspondence should be addressed. Tel: +91 11 26189358; Fax: +91 11 26162316; Email: sunilm{at}icgeb.res.in

Received July 31, 2006. Revised October 11, 2006. Accepted October 13, 2006.

Geminiviruses replicate by rolling circle mode of replication (RCR) and the viral Rep protein initiates RCR by the site-specific nicking at a conserved nonamer (TAATATT{downarrow} AC) sequence. The mechanism of subsequent steps of the replication process, e.g. helicase activity to drive fork-elongation, etc. has largely remained obscure. Here we show that Rep of a geminivirus, namely, Mungbean yellow mosaic India virus (MYMIV), acts as a replicative helicase. The Rep-helicase, requiring ≥6 nt space for its efficient activity, translocates in the 3'->5' direction, and the presence of forked junction in the substrate does not influence the activity to any great extent. Rep forms a large oligomeric complex and the helicase activity is dependent on the oligomeric conformation (~24mer). The role of Rep as a replicative helicase has been demonstrated through ex vivo studies in Saccharomyces cerevisiae and in planta analyses in Nicotiana tabacum. We also establish that such helicase activity is not confined to the MYMIV system alone, but is also true with at least two other begomoviruses, viz., Mungbean yellow mosaic virus (MYMV) and Indian cassava mosaic virus (ICMV).


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Virol.Home page
K. Kittelmann, P. Rau, B. Gronenborn, and H. Jeske
Plant Geminivirus Rep Protein Induces Rereplication in Fission Yeast
J. Virol., July 1, 2009; 83(13): 6769 - 6778.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
G. Arguello-Astorga, J. T. Ascencio-Ibanez, M. B. Dallas, B. M. Orozco, and L. Hanley-Bowdoin
High-Frequency Reversion of Geminivirus Replication Protein Mutants during Infection
J. Virol., October 15, 2007; 81(20): 11005 - 11015.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.