Skip Navigation



Nucleic Acids Research Advance Access published online on November 26, 2007
Nucleic Acids Research, doi:10.1093/nar/gkm1056
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (7442K) Freely available
Right arrow Screen PDF (826K) Freely available
Right arrow Supplementary Data
Right arrowOA All Versions of this Article:
36/2/423    most recent
gkm1056v3
gkm1056v2
gkm1056v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Valgardsdottir, R.
Right arrow Articles by Biamonti, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Valgardsdottir, R.
Right arrow Articles by Biamonti, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Molecular Biology

Transcription of Satellite III non-coding RNAs is a general stress response in human cells

Rut Valgardsdottir1, Ilaria Chiodi1, Manuela Giordano1, Antonio Rossi2, Silvia Bazzini1, Claudia Ghigna1, Silvano Riva1 and Giuseppe Biamonti1,*

1Istituto di Neurobiologia e Medicina Molecolare – Consiglio Nazionale delle Ricerche, Via del Fosso di Fiorano 64, 00143 Roma and 2Istituto di Neurobiologia e Medicina Molecolare – Consiglio Nazionale delle Ricerche Roma

*To whom correspondence should be addressed. Tel: +39 0382 546322; Fax: +39 0382 422286; Email: biamonti{at}igm.cnr.it

Received July 9, 2007. Revised November 7, 2007. Accepted November 9, 2007.

In heat-shocked human cells, heat shock factor 1 activates transcription of tandem arrays of repetitive Satellite III (SatIII) DNA in pericentromeric heterochromatin. Satellite III RNAs remain associated with sites of transcription in nuclear stress bodies (nSBs). Here we use real-time RT-PCR to study the expression of these genomic regions. Transcription is highly asymmetrical and most of the transcripts contain the G-rich strand of the repeat. A low level of G-rich RNAs is detectable in unstressed cells and a 104-fold induction occurs after heat shock. G-rich RNAs are induced by a wide range of stress treatments including heavy metals, UV-C, oxidative and hyper-osmotic stress. Differences exist among stressing agents both for the kinetics and the extent of induction (>100- to 80.000-fold). In all cases, G-rich transcripts are associated with nSBs. On the contrary, C-rich transcripts are almost undetectable in unstressed cells and modestly increase after stress. Production of SatIII RNAs after hyper-osmotic stress depends on the Tonicity Element Binding Protein indicating that activation of the arrays is triggered by different transcription factors. This is the first example of a non-coding RNA whose transcription is controlled by different transcription factors under different growth conditions.

The authors wish it to be known that, in their opinion, the first three authors should be regarded as joint First Authors.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
A. Eymery, B. Horard, M. E. Atifi-Borel, G. Fourel, F. Berger, A.-L. Vitte, A. Van den Broeck, E. Brambilla, A. Fournier, M. Callanan, et al.
A transcriptomic analysis of human centromeric and pericentric sequences in normal and tumor cells
Nucleic Acids Res., October 1, 2009; 37(19): 6340 - 6354.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. Sandqvist, J. K. Bjork, M. Akerfelt, Z. Chitikova, A. Grichine, C. Vourc'h, C. Jolly, T. A. Salminen, Y. Nymalm, and L. Sistonen
Heterotrimerization of Heat-Shock Factors 1 and 2 Provides a Transcriptional Switch in Response to Distinct Stimuli
Mol. Biol. Cell, March 1, 2009; 20(5): 1340 - 1347.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.