Skip Navigation



Nucleic Acids Research Advance Access published online on December 6, 2007
Nucleic Acids Research, doi:10.1093/nar/gkm1081
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (5260K) Freely available
Right arrow Screen PDF (558K) Freely available
Right arrow Supplementary Data
Right arrowOA All Versions of this Article:
36/2/e10    most recent
gkm1081v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Petersen, J.
Right arrow Articles by Dufva, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Petersen, J.
Right arrow Articles by Dufva, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

RNA

Use of a multi-thermal washer for DNA microarrays simplifies probe design and gives robust genotyping assays

Jesper Petersen1, Lena Poulsen2, Sarunas Petronis3, Henrik Birgens1 and Martin Dufva2,*

1Department of Haematology, Herlev Hospital, University of Copenhagen, Herlev Ringvej 75, DK-2730 Herlev, Denmark, 2Microarray Group, Department of Micro and Nanotechnology, Technical University of Denmark, Oersteds Plads, Bld. 345 East, DK-2800 Kongens Lyngby, Denmark and 3Department of Applied Physics, Fysikgården 4, Chalmers University of Technology, SE-412 96 Göteborg, Sweden

*To whom correspondence should be addressed. Tel: +45 4525 6324; Fax: +45 4588 7762; Email: mdu{at}mic.dtu.dk

Received October 4, 2007. Revised November 16, 2007. Accepted November 16, 2007.

DNA microarrays are generally operated at a single condition, which severely limits the freedom of designing probes for allele-specific hybridization assays. Here, we demonstrate a fluidic device for multi-stringency posthybridization washing of microarrays on microscope slides. This device is called a multi-thermal array washer (MTAW), and it has eight individually controlled heating zones, each of which corresponds to the location of a subarray on a slide. Allele-specific oligonucleotide probes for nine mutations in the beta-globin gene were spotted in eight identical subarrays at positions corresponding to the temperature zones of the MTAW. After hybridization with amplified patient material, the slides were mounted in the MTAW, and each subarray was exposed to different temperatures ranging from 22 to 40°C. When processed in the MTAW, probes selected without considering melting temperature resulted in improved genotyping compared with probes selected according to theoretical melting temperature and run under one condition. In conclusion, the MTAW is a versatile tool that can facilitate screening of a large number of probes for genotyping assays and can also enhance the performance of diagnostic arrays.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
L. Poulsen, M. J. Soe, D. Snakenborg, L. B. Moller, and M. Dufva
Multi-stringency wash of partially hybridized 60-mer probes reveals that the stringency along the probe decreases with distance from the microarray surface
Nucleic Acids Res., November 1, 2008; 36(20): e132 - e132.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.