Skip Navigation



Nucleic Acids Research Advance Access published online on December 20, 2007
Nucleic Acids Research, doi:10.1093/nar/gkm1120
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (7134K) Freely available
Right arrow Screen PDF (771K) Freely available
Right arrow Supplementary Data
Right arrowOA All Versions of this Article:
36/3/1037    most recent
gkm1120v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Fuke, H.
Right arrow Articles by Ohno, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fuke, H.
Right arrow Articles by Ohno, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

RNA

Role of poly (A) tail as an identity element for mRNA nuclear export

Hiroyuki Fuke and Mutsuhito Ohno*

Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan, and Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Tokyo 102-0075, Japan

*To whom correspondence should be addressed. Tel: +81 75 751 4018; Fax: +81 75 751 3992; Email: hitoohno{at}virus.kyoto-u.ac.jp

Received September 16, 2007. Revised November 4, 2007. Accepted December 1, 2007.

Different RNA species are rigorously discriminated and exported by distinct export factors, but this discrimination mechanism remains largely unknown. We previously showed, by RNA microinjection experiments, that intronless mRNAs are discriminated from U snRNAs based on their difference in RNA length. However, it was unclear how they are discriminated in the natural situation in which their nascent transcripts emerge progressively during transcription. We hypothesized that transcription from the corresponding promoters is important for this discrimination. Here we show that contrary to our hypothesis, the discrimination process was not significantly influenced by whether transcription occurred from an mRNA- versus a U snRNA-type promoter. Rather, the features of transcribed RNAs determined the RNA identity, consistent with our previous results of RNA microinjection. Moreover, we found that the poly (A) tail can function as an identity element for mRNA export. The presence of a poly (A) tail of an appropriate length committed otherwise short Pol II transcripts to the mRNA export pathway in a dominant manner, indicating that the poly (A) tail either contributes to increasing the RNA length or functions as a platform to recruit mRNA export factors. Our results reveal a novel function of the poly (A) tail in mRNA export.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
X. Qu, S. Lykke-Andersen, T. Nasser, C. Saguez, E. Bertrand, T. H. Jensen, and C. Moore
Assembly of an Export-Competent mRNP Is Needed for Efficient Release of the 3'-End Processing Complex after Polyadenylation
Mol. Cell. Biol., October 1, 2009; 29(19): 5327 - 5338.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.