Skip Navigation



Nucleic Acids Research Advance Access published online on January 10, 2008
Nucleic Acids Research, doi:10.1093/nar/gkm1162
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (3759K) Freely available
Right arrow Screen PDF (693K) Freely available
Right arrow Supplementary Data
Right arrowOA All Versions of this Article:
36/7/2123    most recent
gkm1162v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Chen, J. M.
Right arrow Articles by Liu, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, J. M.
Right arrow Articles by Liu, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2008 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Molecular Biology

Lsr2 of Mycobacterium tuberculosis is a DNA-bridging protein

Jeffrey M. Chen1, Huiping Ren1, James E. Shaw2, Yu Jing Wang1, Ming Li1, Andrea S. Leung1, Vanessa Tran1, Nicolas M. Berbenetz1, Dana Kocíncová3, Christopher M. Yip2, Jean-Marc Reyrat3,4 and Jun Liu1,*

1Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, 2Department of Biochemistry, University of Toronto, Toronto, Ontario M5S 3G9, Canada, 3Université Paris Descartes, Faculté de Médecine René Descartes and 4Inserm, U570, Unité de Pathogénie des Infections Systémiques-Groupe AVENIR, Paris Cedex 15, F-75730, France

*To whom correspondence should be addressed: Tel: 416 946 5067; Fax: 416 978 6885; Email: jun.liu{at}utoronto.ca

Received November 22, 2007. Revised December 12, 2007. Accepted December 13, 2007.

Lsr2 is a small, basic protein present in Mycobacterium and related actinomycetes. Recent studies suggest that Lsr2 is a regulatory protein involved in multiple cellular processes including cell wall biosynthesis and antibiotic resistance. However, the underlying molecular mechanisms remain unknown. In this article, we performed biochemical studies of Lsr2–DNA interactions and structure–function analysis of Lsr2. Analysis by atomic force microscopy revealed that Lsr2 has the ability to bridge distant DNA segments, suggesting that Lsr2 plays a role in the overall organization and compactness of the nucleoid. Mutational analysis identified critical residues and selection of dominant negative mutants demonstrated that both DNA binding and protein oligomerization are essential for the normal functions of Lsr2 in vivo. These results provide strong evidence that Lsr2 is a DNA bridging protein, which represents the first identification of such proteins in bacteria phylogenetically distant from the Enterobacteriaceae. DNA bridging by Lsr2 also provides a mechanism of transcriptional regulation by Lsr2.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Bacteriol.Home page
M. Letek, A. A. Ocampo-Sosa, M. Sanders, U. Fogarty, T. Buckley, D. P. Leadon, P. Gonzalez, M. Scortti, W. G. Meijer, J. Parkhill, et al.
Evolution of the Rhodococcus equi vap Pathogenicity Island Seen through Comparison of Host-Associated vapA and vapB Virulence Plasmids
J. Bacteriol., September 1, 2008; 190(17): 5797 - 5805.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.